验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HSC-T6 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells |
| Positive IHC detected in | human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66587-1-Ig targets DNAJB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2258 Product name: Recombinant human DNAJB6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC000177 Sequence: MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily B, member 6 |
| 别名 | DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2 |
| 计算分子量 | 241 aa, 27 kDa |
| 观测分子量 | 27 kDa, 38 kDa |
| GenBank蛋白编号 | BC000177 |
| 基因名称 | DNAJB6 |
| Gene ID (NCBI) | 10049 |
| RRID | AB_2881947 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O75190 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| IHC protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| WB protocol for DNAJB6 antibody 66587-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC | ||
Biomedicines Abnormally High Expression of DNAJB6 Accelerates Malignant Progression of Lung Adenocarcinoma
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