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验证数据展示

  • WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

    WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66587-1-Ig (same clone as 66587-1-PBS)

    IF Staining of HepG2 using 66587-1-Ig (same clone as 66587-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJB6 antibody (66587-1-Ig, Clone: 1A1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DNAJB6 Monoclonal antibody, PBS Only

DNAJB6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66587-1-PBS

产品说明书

CloneNo. 1A1D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66587-1-PBS targets DNAJB6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DNAJB6 fusion protein Ag2258 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 6
别名 DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2
计算分子量 241 aa, 27 kDa
观测分子量 27 kDa, 38 kDa
GenBank蛋白编号BC000177
基因名称 DNAJB6
Gene ID (NCBI) 10049
RRIDAB_2881947
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75190
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.