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CoraLite® Plus 488-conjugated DNAJB1 Polyclonal antibody
DNAJB1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13174-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-13174 targets DNAJB1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | DNAJB1 fusion protein Ag3830 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 1 |
| Synonyms | DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1 |
| Calculated Molecular Weight | 340 aa, 40 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC019827 |
| Gene Symbol | DNAJB1 |
| Gene ID (NCBI) | 3337 |
| RRID | AB_2919069 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25685 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 DNAJB1 antibody CL488-13174 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
