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验证数据展示

  • IF Staining of HeLa using CL488-10434

    IF Staining of HeLa using CL488-10434

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Smac/DIABLO antibody (CL488-10434) at dilution of 1:200, CL594-phalloidin (red).

  • IF Staining of HepG2 using CL488-10434

    IF Staining of HepG2 using CL488-10434

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Smac/DIABLO antibody (CL488-10434) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-10434

    FC experiment of HeLa using CL488-10434

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DIABLO (CL488-10434) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Smac/DIABLO Polyclonal antibody

Smac/DIABLO Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10434

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

DIABLO, DFNA64, Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein, Diablo IAP-binding mitochondrial protein, cleaved form

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10434-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10434 targets Smac/DIABLO in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0696

Product name: Recombinant human DIABLO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC011909

Sequence: MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diablo homolog (Drosophila)
别名 DIABLO, DFNA64, Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein, Diablo IAP-binding mitochondrial protein, cleaved form
计算分子量 27 kDa
观测分子量 20 kDa
GenBank蛋白编号BC011909
基因名称 DIABLO
Gene ID (NCBI) 56616
RRIDAB_3083877
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR28
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Smac/DIABLO antibody CL488-10434Download protocol
IF protocol for CL Plus 488 Smac/DIABLO antibody CL488-10434Download protocol
Standard Protocols
Click here to view our Standard Protocols
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