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CoraLite® Plus 488-conjugated DHPS Monoclonal antibody

DHPS Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4F5

Cat No : CL488-67998

Print datasheet

Synonyms

deoxyhypusine synthase, DHPS, DHS, DS, MIG13

验证数据展示

  • FC experiment of HeLa using CL488-67998

    FC experiment of HeLa using CL488-67998

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DHPS (CL488-67998, Clone:1E4F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67998-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67998 targets DHPS in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen DHPS fusion protein Ag31046 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name deoxyhypusine synthase
Synonyms deoxyhypusine synthase, DHPS, DHS, DS, MIG13
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49366
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DHPS antibody CL488-67998Download protocol
Standard Protocols
Click here to view our Standard Protocols