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验证数据展示

  • WB analysis using 67998-1-Ig

    WB analysis using 67998-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DHPS Monoclonal antibody

DHPS Monoclonal Antibody for WB, ELISA
Cat No. 67998-1-Ig

产品说明书

CloneNo. 1E4F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

1E4F5, DHS, DS, EC:2.5.1.46, MIG13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67998-1-Ig targets DHPS in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31046

Product name: Recombinant human DHPS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-369 aa of BC014016

Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deoxyhypusine synthase
别名 1E4F5, DHS, DS, EC:2.5.1.46, MIG13
计算分子量 41 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014016
基因名称 DHPS
Gene ID (NCBI) 1725
RRIDAB_2918746
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP49366
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for DHPS antibody 67998-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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