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验证数据展示

  • WB analysis using 86894-1-RR (same clone as 86894-1-PBS)

    WB analysis using 86894-1-RR (same clone as 86894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86894-1-RR (DDX41 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86894-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86894-1-RR (same clone as 86894-1-PBS)

    IF Staining of MCF-7 using 86894-1-RR (same clone as 86894-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DDX41 antibody (86894-1-RR, Clone: 252011G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86894-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02765-1

    Cytometric bead array sample test of MP02765-1

    The mean DDX41 concentration was determined to be 13.3 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load, 4.9 ng/mL in NIH/3T3 cell extract based on a 1.2 mg/mL extract load, 11.1 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02765-1

    Cytometric bead array standard curve of MP02765-1

    Cytometric bead array standard curve of MP02765-1, DDX41 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86894-1-PBS. Detection antibody: 86894-2-PBS. Standard: Ag26593. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 86894-1-RR

    Affinity and Kinetic Characterization of 86894-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86894-1-RR against Human DDX41 were performed. The affinity constant is 0.736 nM.

DDX41 Recombinant monoclonal antibody, PBS Only (Capture)

DDX41 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86894-1-PBS

产品说明书

CloneNo. 252011G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86894-1-PBS targets DDX41 as part of a matched antibody pair:

MP02765-1: 86894-1-PBS capture and 86894-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26593

Product name: Recombinant human DDX41 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-144 aa of BC015476

Sequence: MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
别名 ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC015476
基因名称 DDX41
Gene ID (NCBI) 51428
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJV9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.

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