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验证数据展示

  • WB analysis using 86894-1-RR

    WB analysis using 86894-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86894-1-RR (DDX41 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 86894-1-RR

    IF Staining of MCF-7 using 86894-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DDX41 antibody (86894-1-RR, Clone: 252011G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86894-1-RR

    Affinity and Kinetic Characterization of 86894-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86894-1-RR against Human DDX41 were performed. The affinity constant is 0.736 nM.

DDX41 Recombinant monoclonal antibody

DDX41 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86894-1-RR

产品说明书

CloneNo. 252011G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells, THP-1 cells, RAW 264.7 cells, PC-12 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86894-1-RR targets DDX41 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26593

Product name: Recombinant human DDX41 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-144 aa of BC015476

Sequence: MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
别名 ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC015476
基因名称 DDX41
Gene ID (NCBI) 51428
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.

实验方案

Product Specific Protocols
IF protocol for DDX41 antibody 86894-1-RRDownload protocol
WB protocol for DDX41 antibody 86894-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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