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验证数据展示

  • WB analysis using 14916-1-AP (same clone as 14916-1-PBS)

    WB analysis using 14916-1-AP (same clone as 14916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 14916-1-AP (same clone as 14916-1-PBS)

    WB analysis of A549 using 14916-1-AP (same clone as 14916-1-PBS)

    WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDOST antibody included
  • IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDOST antibody included
  • IP experiment of mouse pancreas using 14916-1-AP (same clone as 14916-1-PBS)

    IP experiment of mouse pancreas using 14916-1-AP (same clone as 14916-1-PBS)

    IP result of anti-DDOST (IP:14916-1-AP, 4ug; Detection:14916-1-AP 1:1000) with mouse pancreas tissue lysate 3200 ug. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDOST Polyclonal antibody, PBS Only

DDOST Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14916-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, KIAA0115, OK/SW cl.45, OK/SW-cl.45, OST48

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® DDOST Ready-To-Use IHC Kit. DDOST primary antibody included.

产品信息

14916-1-PBS targets DDOST in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6727

Product name: Recombinant human DDOST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 92-433 aa of BC002594

Sequence: FLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dolichyl-diphosphooligosaccharide-protein glycosyltransferase
别名 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, KIAA0115, OK/SW cl.45, OK/SW-cl.45, OST48
计算分子量 49 kDa
观测分子量 48 kDa
GenBank蛋白编号BC002594
基因名称 DDOST
Gene ID (NCBI) 1650
RRIDAB_2230534
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39656
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDOST (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit), also known as OST48, is an integral membrane protein and a core component of the oligosaccharyltransferase (OST) complex located in the endoplasmic reticulum (ER). It plays a critical role in the transfer of pre-assembled oligosaccharide chains to asparagine residues of nascent polypeptides, a fundamental process known as N-linked protein glycosylation. Beyond its enzymatic function, DDOST contributes to protein folding quality control, endoplasmic reticulum-associated degradation (ERAD), and the unfolded protein response (UPR). Altered expression of DDOST has been implicated in cancer progression, metastasis, and chemoresistance.

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