• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DDOST Polyclonal antibody

DDOST Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14916-1-AP

Print datasheet

Synonyms

AGE R1, DDOST, DDOST 48 kDa subunit, KIAA0115, OK/SW cl.45, OST, OST48, WBP1

验证数据展示

  • WB analysis of A549 using 14916-1-AP

    WB analysis of A549 using 14916-1-AP

    WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells.

  • IHC staining of mouse testis using 14916-1-AP

    IHC staining of mouse testis using 14916-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14916-1-AP

    WB analysis using 14916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14916-1-AP

    IHC staining of mouse testis using 14916-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse pancreas using 14916-1-AP

    IP experiment of mouse pancreas using 14916-1-AP

    IP result of anti-DDOST (IP:14916-1-AP, 4ug; Detection:14916-1-AP 1:1000) with mouse pancreas tissue lysate 3200 ug.

经过测试的应用

Positive WB detected inU-87 MG cells, A549 cells, HeLa cells, mouse pancreas, rat pancreas
Positive IP detected inmouse pancreas tissue
Positive IHC detected inmouse testis tissue, rat small intestine tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14916-1-AP targets DDOST in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DDOST fusion protein Ag6727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Synonyms AGE R1, DDOST, DDOST 48 kDa subunit, KIAA0115, OK/SW cl.45, OST, OST48, WBP1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002594
Gene Symbol DDOST
Gene ID (NCBI) 1650
RRIDAB_2230534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP39656
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDOST antibody 14916-1-APDownload protocol
IHC protocol for DDOST antibody 14916-1-APDownload protocol
IP protocol for DDOST antibody 14916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks
View Article
mouseWB,CoIP

Res Sq

Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction

Authors - Hossein Ardehali
View Article
humanIHC

IET Syst Biol

An immune-related multi-omics analysis of dolichyl-diphosphooligosaccharide protein glycosyltransferase in glioma: Prognostic value exploration and competitive endogenous RNA network identification

Authors - Jie Liu
View Article
humanIHC

J Hepatocell Carcinoma

Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma

Authors - Shusheng Lin
View Article