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CoraLite® Plus 488-conjugated DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-22373

Print datasheet

Synonyms

DCP1A, FLJ21691, HSA275986, mRNA decapping enzyme 1A, Nbla00360, SMAD4IP1, SMIF, Transcription factor SMIF

验证数据展示

  • IF Staining of HepG2 using CL488-22373

    IF Staining of HepG2 using CL488-22373

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DCP1A antibody (CL488-22373) at dilution of 1:100.

  • FC experiment of HeLa using CL488-22373

    FC experiment of HeLa using CL488-22373

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DCP1A (CL488-22373) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22373-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22373 targets DCP1A in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen DCP1A fusion protein Ag18027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DCP1 decapping enzyme homolog A (S. cerevisiae)
Synonyms DCP1A, FLJ21691, HSA275986, mRNA decapping enzyme 1A, Nbla00360, SMAD4IP1, SMIF, Transcription factor SMIF
Calculated Molecular Weight 582 aa, 63 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007439
Gene Symbol DCP1A
Gene ID (NCBI) 55802
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPI6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
FC protocol for CL Plus 488 DCP1A antibody CL488-22373Download protocol
Standard Protocols
Click here to view our Standard Protocols