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验证数据展示

  • WB analysis using 22373-1-AP

    WB analysis using 22373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 22373-1-AP

    IP experiment of mouse brain using 22373-1-AP

    IP result of anti-DCP1A (IP:22373-1-AP, 4ug; Detection:22373-1-AP 1:3000) with mouse brain tissue lysate 3200ug.

  • WB analysis of HEK-293 using 22373-1-AP

    WB analysis of HEK-293 using 22373-1-AP

    WB result of DCP1A antibody (22373-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCP1A transfected HEK-293 cells.

  • IHC staining of mouse lung using 22373-1-AP

    IHC staining of mouse lung using 22373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCP1A antibody included
  • IHC staining of mouse lung using 22373-1-AP

    IHC staining of mouse lung using 22373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCP1A antibody included
  • IF Staining of HepG2 using 22373-1-AP

    IF Staining of HepG2 using 22373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 22373-1-AP

    IF Staining of HeLa using 22373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HeLa using 22373-1-AP

    FC experiment of HeLa using 22373-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DCP1A (22373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

DCP1A Polyclonal antibody

DCP1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 22373-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22373-1-AP targets DCP1A in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IF, IP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag18027

Product name: Recombinant human DCP1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC007439

Sequence: MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360
计算分子量 582 aa, 63 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
RRIDAB_2879092
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NPI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

实验方案

Product Specific Protocols
FC protocol for DCP1A antibody 22373-1-APDownload protocol
IF protocol for DCP1A antibody 22373-1-APDownload protocol
IHC protocol for DCP1A antibody 22373-1-APDownload protocol
IP protocol for DCP1A antibody 22373-1-APDownload protocol
WB protocol for DCP1A antibody 22373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Targeted stress granule regulation by engineering a non-catalytic O-GlcNAc transferase.

Authors - Na Wang
View Article
humanWB

Mol Cell

The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance

Authors - Tao Liu
View Article
mouseWB

Adv Sci (Weinh)

Enhanced Protein Synthesis and Hippocampus-Dependent Memory via Inhibition of YTHDF2-Mediated m(6)A mRNA Degradation.

Authors - Kuan Li
View Article

Cell Mol Biol Lett

CCHCR1 links P-body proteins to the centrosome and is required for ciliogenesis through interacting with OFD1 and PCM1

Authors - Junshan Zhang
View Article
WB

Dev Cell

A lineage-specific selective autophagy receptor module mediates P-body turnover.

Authors - Alibek Abdrakhmanov
View Article
ratIF

EMBO J

Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neurons.

Authors - Yu Feng
View Article
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