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验证数据展示

  • IF Staining of mouse brain using CL488-29800

    IF Staining of mouse brain using CL488-29800

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCLK1 antibody (CL488-29800) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL488-29800

    IF Staining of mouse brain using CL488-29800

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCLK1 antibody (CL488-29800) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Neuro-2a using CL488-29800

    FC experiment of Neuro-2a using CL488-29800

    1x10^6 Neuro-2a cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Dclk1 Polyclonal Antibody (CL488-29800)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated DCLK1 Polyclonal antibody

DCLK1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-29800

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

IF-P, FC (Intra)

别名

DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inNeuro-2a cells
Positive FC detected inNeuro-2a cells
For other applications, we recommend the unconjugated version of this antibody, 29800-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29800 targets DCLK1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag32007

Product name: Recombinant human DCLK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 648-740 aa of NM_004734

Sequence: NDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 doublecortin-like kinase 1
别名 DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369
计算分子量82KD
观测分子量48 kDa, 82 kDa
GenBank蛋白编号NM_004734
基因名称 DCLK1
Gene ID (NCBI) 9201
RRIDAB_3672840
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCLK1 antibody CL488-29800Download protocol
Standard Protocols
Click here to view our Standard Protocols