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验证数据展示

  • IHC staining of human colon cancer using 29800-1-AP

    IHC staining of human colon cancer using 29800-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 29800-1-AP

    IP experiment of mouse brain using 29800-1-AP

    IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug.

  • WB analysis using 29800-1-AP

    WB analysis using 29800-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29800-1-AP

    IHC staining of human colon cancer using 29800-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 29800-1-AP

    IHC staining of human pancreas cancer using 29800-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 29800-1-AP

    IHC staining of human pancreas cancer using 29800-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 29800-1-AP

    IF Staining of mouse brain using 29800-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 29800-1-AP

    IF Staining of mouse brain using 29800-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DCLK1 Polyclonal antibody

DCLK1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 29800-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

DCAMKL1, DCDC3A, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29800-1-AP targets DCLK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32007

Product name: Recombinant human DCLK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 648-740 aa of NM_004734

Sequence: NDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 doublecortin-like kinase 1
别名 DCAMKL1, DCDC3A, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1
计算分子量82KD
观测分子量48 kDa, 82 kDa
GenBank蛋白编号NM_004734
基因名称 DCLK1
Gene ID (NCBI) 9201
RRIDAB_3086165
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15075
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

实验方案

Product Specific Protocols
WB protocol for DCLK1 antibody 29800-1-APDownload protocol
IHC protocol for DCLK1 antibody 29800-1-APDownload protocol
IF protocol for DCLK1 antibody 29800-1-APDownload protocol
IP protocol for DCLK1 antibody 29800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols