验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
67203-1-Ig targets DCC-Specific in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | mouse |
| 免疫原 | DCC-Specific fusion protein Ag20569 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | deleted in colorectal carcinoma |
| 别名 | Colorectal cancer suppressor, CRC18, CRCR1, DCC, IGDCC1, Netrin receptor DCC, Tumor suppressor protein DCC |
| 计算分子量 | 1447 aa, 158 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | NM_005215 |
| 基因名称 | DCC |
| Gene ID (NCBI) | 1630 |
| RRID | AB_2882496 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43146 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC plays a key role in the developing nervous system. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCC-Specific antibody 67203-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses | ||
Neurobiol Stress DCC, a potential target for controlling fear memory extinction and hippocampal LTP in male mice receiving single prolonged stress |