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验证数据展示

  • FC experiment of Transfected HEK-293T using 98205-1-RR

    FC experiment of Transfected HEK-293T using 98205-1-RR

    1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone: 241683G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected HEK-293T using 98205-1-RR

    FC experiment of Transfected HEK-293T using 98205-1-RR

    1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (98205-1-RR, Clone:241683G8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98205-1-RR

    Affinity and Kinetic Characterization of 98205-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98205-1-RR against Human DC-SIGNR/CD299 were performed. The affinity constant is 3.68 nM.

Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody

DC-SIGNR/CD299 Recombinant Antibody for FC
Cat No. 98205-1-RR

产品说明书

CloneNo. 241683G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CLEC4M, CD299, CD209L1, CD209L, CD209 antigen-like protein 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inTransfected HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy DC-SIGNR/CD299 Ready-To-Use IHC Kit. DC-SIGNR/CD299 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98205-1-RR targets DC-SIGNR/CD299 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-type lectin domain family 4, member M
别名 CLEC4M, CD299, CD209L1, CD209L, CD209 antigen-like protein 1
计算分子量 45 kDa
GenBank蛋白编号BC038851
基因名称 DC-SIGNR/CD299
Gene ID (NCBI) 10332
RRIDAB_3672336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H2X3
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN/CD209) and DC-SIGN related (DC-SIGNR/CD299/L-SIGN/CD209L/CLEC4M) are trans-membrane C-type lectins that are calcium-dependent carbohydrate-binding proteins, which can act as cell-adhesion and pathogen-recognition receptors (PMID: 18458800). DC-SIGN and DC-SIGNR share 77% amino acid identity (PMID: 10975799; 11226297). DC-SIGN is expressed on subsets of dendritic cells and macrophages while DC-SIGNR is restricted to endothelial cells of the liver, lymph nodes and placenta (PMID: 17509452). DC-SIGNR servers as a receptor for ICAM-3. It recognizes a wide range of evolutionarily divergent pathogens.

实验方案

Product Specific Protocols
FC protocol for DC-SIGNR/CD299 antibody 98205-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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