验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 28506-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-28506 targets Cytokeratin 5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25436 Product name: Recombinant human KRT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-60 aa of BC024292 Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 5 |
| 别名 | CK 5, CK-5, keratin 5, KRT5, 58 kDa cytokeratin |
| 计算分子量 | 590 aa, 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC024292 |
| 基因名称 | Cytokeratin 5 |
| Gene ID (NCBI) | 3852 |
| RRID | AB_2934968 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13647 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Cytokeratin 5 antibody CL647-28506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
