验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-28506 targets Cytokeratin 5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25436 Product name: Recombinant human KRT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-60 aa of BC024292 Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 5 |
| 别名 | CK 5, CK-5, keratin 5, KRT5, 58 kDa cytokeratin |
| 计算分子量 | 590 aa, 62 kDa |
| GenBank蛋白编号 | BC024292 |
| 基因名称 | Cytokeratin 5 |
| Gene ID (NCBI) | 3852 |
| RRID | AB_2919650 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 激发激光 | Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13647 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 Cytokeratin 5 antibody CL555-28506 | Download protocol |
| IF protocol for CL555 Cytokeratin 5 antibody CL555-28506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
