验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HT-29 cells |
| Positive FC (Intra) detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-17329 targets Cytokeratin 20 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, rabbit |
| 免疫原 |
CatNo: Ag11308 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 20 |
| 别名 | KRT20, CK 20, CK20, CK-20, Cytokeratin-20 |
| 计算分子量 | 424 aa, 48 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC031559 |
| 基因名称 | Cytokeratin 20 |
| Gene ID (NCBI) | 54474 |
| RRID | AB_3084003 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35900 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329 | Download protocol |
| IF protocol for CL Plus 488 Cytokeratin 20 antibody CL488-17329 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
