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验证数据展示

  • WB analysis using 17329-1-AP

    WB analysis using 17329-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17329-1-AP

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colon using 17329-1-AP

    IHC staining of human colon using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human appendicitis using 17329-1-AP

    IHC staining of human appendicitis using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human appendicitis using 17329-1-AP

    IHC staining of human appendicitis using 17329-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17329-1-AP (Cytokeratin 20 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of Rabbit colon using 17329-1-AP

    IF Staining of Rabbit colon using 17329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Rabbit colon tissue using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Caco-2 using 17329-1-AP

    IF Staining of Caco-2 using 17329-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Cytokeratin 20 antibody (17329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HT-29 using 17329-1-AP

    FC experiment of HT-29 using 17329-1-AP

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 20 (17329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Cytokeratin 20 Polyclonal antibody

Cytokeratin 20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 17329-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HT-29 cells
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human colon cancer tissue, human stomach cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inRabbit colon tissue
Positive IF/ICC detected inCaco-2 cells, HeLa cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17329-1-AP targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, rabbit
文献引用反应性human, mouse
免疫原

CatNo: Ag11308

Product name: Recombinant human KRT20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 74-424 aa of BC031559

Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 20
别名 KRT20, CK 20, CK20, CK-20, Cytokeratin-20
计算分子量 424 aa, 48 kDa
观测分子量 46 kDa
GenBank蛋白编号BC031559
基因名称 Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_2133592
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
IHC protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
WB protocol for Cytokeratin 20 antibody 17329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Res

Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons.

Authors - Pingping Zhu
View Article
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
View Article

Neuron

5-hydroxytryptamine produced by enteric serotonergic neurons initiates colorectal cancer stem cell self-renewal and tumorigenesis.

Authors - Pingping Zhu
View Article
humanIF

Biomaterials

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium.

Authors - Yuli Wang
View Article
mouseIF

Cancer Lett

LGR4 cooperates with PrPc to endow the stemness of colorectal cancer stem cells contributing to tumorigenesis and liver metastasis.

Authors - Qi Cheng
View Article
mouseIHC

Nutrients

Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment

Authors - Ming-Hung Shen
View Article
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