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验证数据展示

  • FC experiment of HepG2 using CL750-83276

    FC experiment of HepG2 using CL750-83276

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 Cytochrome C Recombinant Antibody (CL750-83276, Clone:230503E10)(red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL750-83276

    WB analysis using CL750-83276

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83276 (Cytochrome c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  • KD/KO验证
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CoraLite® Plus 750-conjugated Cytochrome c Recombinant monoclonal antibody

Cytochrome c Uni-rAb® Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83276

产品说明书

CloneNo. 230503E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra)

别名

CYCS, CYC, 230503E10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83276-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83276 targets Cytochrome c in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1455

Product name: Recombinant human Cytochrome c protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-105 aa of BC009578

Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c, somatic
别名 CYCS, CYC, 230503E10
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC009578
基因名称 Cytochrome c
Gene ID (NCBI) 54205
RRIDAB_3673778
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP99999
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
FC protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols