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CoraLite® Plus 750-conjugated Cytochrome c Recombinant antibody

Cytochrome c Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83276

产品说明书

CloneNo. 230503E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra)

CYCS, CYC, 230503E10

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83276-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83276 targets Cytochrome c in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Cytochrome c fusion protein Ag1455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c, somatic
别名 CYCS, CYC, 230503E10
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC009578
基因名称 Cytochrome c
Gene ID (NCBI) 54205
RRIDAB_3673778
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP99999
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
FC protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols
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