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验证数据展示

  • WB analysis using 83276-1-RR

    WB analysis using 83276-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83276-1-RR

    WB analysis of HepG2 using 83276-1-RR

    WB result of Cytochrome c antibody (83276-1-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.

  • FC experiment of HeLa using 83276-1-RR

    FC experiment of HeLa using 83276-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 83276-1-RR

    IF Staining of A431 using 83276-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cytochrome c antibody (83276-1-RR, Clone: 230503E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

Cytochrome c Recombinant monoclonal antibody

Cytochrome c Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83276-1-RR

产品说明书

CloneNo. 230503E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

230503E10, CYC, CYCS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse kidney tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83276-1-RR targets Cytochrome c in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1455

Product name: Recombinant human Cytochrome c protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-105 aa of BC009578

Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c, somatic
别名 230503E10, CYC, CYCS
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC009578
基因名称 Cytochrome c
Gene ID (NCBI) 54205
RRIDAB_3670946
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP99999
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
WB protocol for Cytochrome c antibody 83276-1-RRDownload protocol
IF protocol for Cytochrome c antibody 83276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols