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Cytochrome c Recombinant antibody

Cytochrome c Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat No : 83276-1-RR

Print datasheet

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

验证数据展示

  • WB analysis using 83276-1-RR

    WB analysis using 83276-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83276-1-RR

    FC experiment of HeLa using 83276-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, mouse kidney tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83276-1-RR is Cytochrome c Fusion Protein expressed in E. coli.

Tested Applications WB, FC, ELISA
Tested Reactivity Human, Mouse
Immunogen Cytochrome c fusion protein Ag1455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome c, somatic
Synonyms CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC009578
Gene Symbol CYCS
Gene ID (NCBI) 54205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP99999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
WB protocol for Cytochrome c antibody 83276-1-RRDownload protocol
FC protocol for Cytochrome c antibody 83276-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols