Cytochrome c Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230503E10

Cat No : 83276-1-PBS

Synonyms

CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4

WB analysis using 83276-1-RR (same clone as 83276-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83276-1-RR (Cytochrome c antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 83276-1-RR (same clone as 83276-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytochrome c Recombinant antibody (83276-1-RR, Clone:230503E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83276-1-PBS in a different storage buffer formulation.

Size

Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.

The immunogen of 83276-1-PBS is Cytochrome c Fusion Protein expressed in E. coli.

Tested Applications	WB, FC, Indirect ELISA
Tested Reactivity	Human
Immunogen	Cytochrome c fusion protein Ag1455 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	cytochrome c, somatic
Synonyms	CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4
Calculated Molecular Weight	12 kDa
GenBank Accession Number	BC009578
Gene Symbol	CYCS
Gene ID (NCBI)	54205
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P99999
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.