验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells, U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84980-1-RR targets Cyclin T1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | cyclin T1 |
别名 | CCNT1, Cyclin-T1, Cyclin-T, Cyclin T, CCNT |
计算分子量 | 81 kDa |
观测分子量 | 75-81 kDa |
GenBank蛋白编号 | NM_001240 |
基因名称 | Cyclin T1 |
Gene ID (NCBI) | 904 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | O60563 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.
实验方案
Product Specific Protocols | |
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WB protocol for Cyclin T1 antibody 84980-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |