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验证数据展示

  • ChIP experiment of HCT 116 using 84980-1-RR

    ChIP experiment of HCT 116 using 84980-1-RR

    Chromatin was prepared from HCT 116 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Cyclin T1 (84980-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR

  • WB analysis using 84980-1-RR

    WB analysis using 84980-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84980-1-RR (Cyclin T1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 84980-1-RR

    FC experiment of MCF-7 using 84980-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of U2OS using 84980-1-RR

    FC experiment of U2OS using 84980-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Cyclin T1 Recombinant antibody

Cyclin T1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ChIP, ELISA
Cat No. 84980-1-RR

产品说明书

CloneNo. 242256C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ChIP, ELISA

别名

CCNT1, 242256C3, CCNT, Cyclin T, Cyclin-T

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, U2OS cells
Positive ChIP detected inHCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84980-1-RR targets Cyclin T1 in WB, FC (Intra), ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ChIP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin T1
别名 CCNT1, 242256C3, CCNT, Cyclin T, Cyclin-T
计算分子量 81 kDa
观测分子量 75-81 kDa
GenBank蛋白编号NM_001240
基因名称 Cyclin T1
Gene ID (NCBI) 904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60563
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

实验方案

Product Specific Protocols
WB protocol for Cyclin T1 antibody 84980-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols