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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84980-1-RR (same clone as 84980-1-PBS)

    WB analysis using 84980-1-RR (same clone as 84980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84980-1-RR (Cyclin T1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84980-1-RR (same clone as 84980-1-PBS)

    FC experiment of MCF-7 using 84980-1-RR (same clone as 84980-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84980-1-RR (same clone as 84980-1-PBS)

    FC experiment of U2OS using 84980-1-RR (same clone as 84980-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84980-1-PBS in a different storage buffer formulation.

Cyclin T1 Recombinant antibody, PBS Only

Cyclin T1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84980-1-PBS

产品说明书

CloneNo. 242256C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

CCNT1, Cyclin-T1, Cyclin-T, Cyclin T, CCNT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84980-1-PBS targets Cyclin T1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin T1
别名 CCNT1, Cyclin-T1, Cyclin-T, Cyclin T, CCNT
计算分子量 81 kDa
观测分子量 75-81 kDa
GenBank蛋白编号NM_001240
基因名称 Cyclin T1
Gene ID (NCBI) 904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60563
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.