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验证数据展示

  • WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

    WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

Complement factor B Monoclonal antibody, PBS Only

Complement factor B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66154-1-PBS

产品说明书

CloneNo. 8D2B6

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

CFB, 8D2B6, BF, BFD, C3/C5 convertase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

66154-1-PBS targets Complement factor B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19107

Product name: Recombinant human CFB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 574-762 aa of BC007990

Sequence: DYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLG

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 complement factor B
别名 CFB, 8D2B6, BF, BFD, C3/C5 convertase
计算分子量 86 kDa
观测分子量 93 kDa
GenBank蛋白编号BC007990
基因名称 Complement factor B
Gene ID (NCBI) 629
RRIDAB_2881550
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP00751
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

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