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验证数据展示

  • WB analysis of human plasma using 66154-1-Ig

    WB analysis of human plasma using 66154-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of human liver using 66154-1-Ig

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver using 66154-1-Ig

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver using 66154-1-Ig

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 66154-1-Ig

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens).

Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66154-1-Ig

产品说明书

CloneNo. 8D2B6

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

CFB, 8D2B6, BF, BFD, C3/C5 convertase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66154-1-Ig targets Complement factor B in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19107

Product name: Recombinant human CFB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 574-762 aa of BC007990

Sequence: DYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLG

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 complement factor B
别名 CFB, 8D2B6, BF, BFD, C3/C5 convertase
计算分子量 86 kDa
观测分子量 93 kDa
GenBank蛋白编号BC007990
基因名称 Complement factor B
Gene ID (NCBI) 629
RRIDAB_2881550
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP00751
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

实验方案

Product Specific Protocols
IF protocol for Complement factor B antibody 66154-1-IgDownload protocol
IHC protocol for Complement factor B antibody 66154-1-IgDownload protocol
WB protocol for Complement factor B antibody 66154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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