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验证数据展示

  • IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL11A1 antibody included
  • IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    IHC staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL11A1 antibody included
  • WB analysis using 21841-1-AP (same clone as 21841-1-PBS)

    WB analysis using 21841-1-AP (same clone as 21841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL11A1 antibody included
  • IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    IHC staining of human stomach cancer using 21841-1-AP (same clone as 21841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL11A1 antibody included
  • IF Staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    IF Staining of human placenta using 21841-1-AP (same clone as 21841-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21841-1-PBS in a different storage buffer formulation.

Collagen Type XI Polyclonal antibody, PBS Only

Collagen Type XI Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21841-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy COL11A1 Ready-To-Use IHC Kit. COL11A1 primary antibody included.

产品信息

21841-1-PBS targets Collagen Type XI in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16509

Product name: Recombinant human COL11A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1429-1690 aa of BC117697

Sequence: PLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSYDKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFGFEVGPVCFLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type XI, alpha 1
别名 COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain
计算分子量 1818 aa, 182 kDa
观测分子量250 kDa, 181 kDa
GenBank蛋白编号BC117697
基因名称 COL11A1
Gene ID (NCBI) 1301
RRIDAB_2918074
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12107
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)

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