验证数据展示
经过测试的应用
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 3 publications below |
产品信息
21841-1-AP targets Collagen Type XI in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Collagen Type XI fusion protein Ag16509 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type XI, alpha 1 |
| 别名 | COL11A1, COL11, Collagen alpha-1(XI) chain, Collagen alpha 1(XI) chain, COLL6 |
| 计算分子量 | 1818 aa, 182 kDa |
| GenBank蛋白编号 | BC117697 |
| 基因名称 | COL11A1 |
| Gene ID (NCBI) | 1301 |
| RRID | AB_2918074 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12107 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Collagen Type XI antibody 21841-1-AP | Download protocol |
| IF protocol for Collagen Type XI antibody 21841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer | ||
J Orthop Res Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration | ||
BMC Med Genomics Analysis of cancer-associated fibroblasts related genes identifies COL11A1 associated with lung adenocarcinoma prognosis | ||
Front Immunol COL11A1 as an novel biomarker for breast cancer with machine learning and immunohistochemistry validation | ||