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验证数据展示

  • IF Staining of human liver cancer using CL594-66824

    IF Staining of human liver cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL594-66824

    IF Staining of human liver cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66824

    IF Staining of human colon cancer using CL594-66824

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

CoraLite®594-conjugated COL6A1 Monoclonal antibody

COL6A1 Monoclonal Antibody for IF-P
Cat No. CL594-66824

产品说明书

CloneNo. 3H10B1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P

别名

COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue, human colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66824-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66824 targets COL6A1 in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag11809

Product name: Recombinant human COL6A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 770-1028 aa of BC052575

Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type VI, alpha 1
别名 COL6, 3H10B1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain
计算分子量 1028 aa, 109 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC052575
基因名称 COL6A1
Gene ID (NCBI) 1291
RRIDAB_2920045
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12109
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
IF protocol for CL594 COL6A1 antibody CL594-66824Download protocol
Standard Protocols
Click here to view our Standard Protocols