验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy COL2A1 Ready-To-Use IHC Kit. COL2A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28459-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-28459 targets Collagen Type II in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type II, alpha 1 |
| 别名 | COL2, COL2A1, COL11A3, Chondrocalcin, AOM |
| 计算分子量 | 134 kDa |
| GenBank蛋白编号 | BC007252 |
| 基因名称 | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_3672827 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02458 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Collagen Type II antibody CL488-28459 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
