Collagen Type II Polyclonal antibody
Collagen Type II Polyclonal Antibody for IF-P, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (5)
Applications
IF-P, IHC, ELISA and More (2)
Conjugate
Unconjugated
193
验证数据展示
经过测试的应用
Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human lung cancer tissue |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28459-1-AP targets Collagen Type II in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF-P, IHC, ELISA |
Cited Applications | WB, IF, IHC, CoIP |
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat, rabbit, pig |
Immunogen | Collagen Type II fusion protein Ag29265 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | collagen, type II, alpha 1 |
Synonyms | Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Col2α1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC |
Calculated Molecular Weight | 134 kDa |
GenBank Accession Number | BC007252 |
Gene Symbol | COL2A1 |
Gene ID (NCBI) | 1280 |
RRID | AB_2881147 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02458 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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IHC protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
IF protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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Adv Mater Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy | ||
Nat Commun Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. | ||
J Musculoskelet Neuronal Interact Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway | ||
Acta Biomater The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage. | ||
J Nanobiotechnology Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy |