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CoraLite®555-conjugated Chromogranin A Monoclonal antibody

Chromogranin A Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H11C11

Cat No : CL555-60135

Print datasheet

Synonyms

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

验证数据展示

  • IF Staining of PC-12 using CL555-60135

    IF Staining of PC-12 using CL555-60135

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

  • IF Staining of SH-SY5Y using CL555-60135

    IF Staining of SH-SY5Y using CL555-60135

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

  • IF Staining of Neuro-2a using CL555-60135

    IF Staining of Neuro-2a using CL555-60135

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL555-60135

    FC experiment of SH-SY5Y using CL555-60135

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Neuro-2a using CL555-60135

    FC experiment of Neuro-2a using CL555-60135

    1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inPC-12 cells, SH-SY5Y cells, Neuro-2a cells
Positive FC detected inSH-SY5Y cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-60135 targets Chromogranin A in IF, FC (Intra) applications and shows reactivity with human, rat, pig, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, rat, pig, mouse
Immunogen Chromogranin A fusion protein Ag0807 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromogranin A (parathyroid secretory protein 1)
Synonyms CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2
Calculated Molecular Weight 51 kDa
GenBank Accession NumberBC006459
Gene Symbol CHGA
Gene ID (NCBI) 1113
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10645
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
IF protocol for CL555 Chromogranin A antibody CL555-60135Download protocol
FC protocol for CL555 Chromogranin A antibody CL555-60135Download protocol
Standard Protocols
Click here to view our Standard Protocols