Chk1 Monoclonal antibody
Chk1 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K562 cells, K-562 |
| Positive IHC detected in | Prostate cancer tissue, human prostate cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65016-1-Ig targets Chk1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | Recombinant Protein 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CHK1 checkpoint homolog (S. pombe) |
| Synonyms | CHEK1, Chk1 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC004202 |
| Gene Symbol | CHEK1 |
| Gene ID (NCBI) | 1111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14757 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at 2-8°C. |
背景介绍
In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases.
发表文章
| Species | Application | Title |
|---|---|---|