验证数据展示
经过测试的应用
| Positive WB detected in | K562 cells, K-562 |
| Positive IHC detected in | Prostate cancer tissue, human prostate cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65016-1-Ig targets Chk1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CHK1 checkpoint homolog (S. pombe) |
| 别名 | CHEK1, Chk1 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC004202 |
| 基因名称 | Chk1 |
| Gene ID (NCBI) | 1111 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O14757 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at 2-8°C. |
背景介绍
In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases.