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验证数据展示

  • IF Staining of HeLa using CL488-10252

    IF Staining of HeLa using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • IF Staining of U2OS using CL488-10252

    IF Staining of U2OS using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for IF/ICC
Cat No. CL488-10252

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

bA276H19.3, CDC20, CDC20A, p55CDC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy CDC20 Ready-To-Use IHC Kit. CDC20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10252-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10252 targets Cdc20 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag0295

Product name: Recombinant human CDC20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-211 aa of BC001088

Sequence: MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 20 homolog (S. cerevisiae)
别名 bA276H19.3, CDC20, CDC20A, p55CDC
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC001088
基因名称 CDC20
Gene ID (NCBI) 991
RRIDAB_3083867
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12834
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cdc20 antibody CL488-10252Download protocol
Standard Protocols
Click here to view our Standard Protocols