CoraLite® Plus 488-conjugated Catalase Monoclonal antibody
Catalase Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F8D7
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66765-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66765 targets Catalase in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | Catalase fusion protein Ag16916 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | catalase |
| Synonyms | CAT, catalase, Peroxisome Marker |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC112219 |
| Gene Symbol | CAT |
| Gene ID (NCBI) | 847 |
| RRID | AB_2919364 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 488 nm / 515 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P04040 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Catalase antibody CL488-66765 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |