CoraLite® Plus 488-conjugated Calsequestrin 2 Monoclonal antibody
Calsequestrin 2 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, pig, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C10A1
验证数据展示
经过测试的应用
| Positive IF detected in | mouse heart tissue |
For other applications, we recommend the unconjugated version of this antibody, 66419-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66419 targets Calsequestrin 2 in IF applications and shows reactivity with human, rat, pig, mouse samples.
| Tested Applications | IF-P |
| Tested Reactivity | human, rat, pig, mouse |
| Immunogen | Calsequestrin 2 fusion protein Ag13246 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | calsequestrin 2 (cardiac muscle) |
| Synonyms | Calsequestrin 2, CASQ2, FLJ26321, FLJ93514, PDIB2 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC022288 |
| Gene Symbol | CASQ2 |
| Gene ID (NCBI) | 845 |
| RRID | AB_2919322 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14958 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Calsequestrin 2 antibody CL488-66419 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |