验证数据展示
经过测试的应用
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-66496 targets Calretinin in IHC applications and shows reactivity with Human, mouse, pig, rat samples.
| 经测试应用 | IHC Application Description |
| 经测试反应性 | Human, mouse, pig, rat |
| 免疫原 |
CatNo: Ag2924 Product name: Recombinant human Calretinin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC015484 Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | calbindin 2 |
| 别名 | 29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR |
| 计算分子量 | 29 kDa |
| GenBank蛋白编号 | BC015484 |
| 基因名称 | Calretinin |
| Gene ID (NCBI) | 794 |
| RRID | AB_2883074 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22676 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin Calretinin antibody Biotin-66496 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
