• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13938

Print datasheet

Synonyms

Basic calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

验证数据展示

  • IF Staining of C2C12 using CL488-13938

    IF Staining of C2C12 using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

经过测试的应用

Positive IF detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 13938-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13938 targets Calponin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Calponin fusion protein Ag4989 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 1, basic, smooth muscle
Synonyms Basic calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC022015
Gene Symbol CNN1
Gene ID (NCBI) 1264
RRIDAB_2919081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols