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验证数据展示

  • IF Staining of C2C12 using CL488-13938

    IF Staining of C2C12 using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

  • IF Staining of mouse heart using CL488-13938

    IF Staining of mouse heart using CL488-13938

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MDCK using CL488-13938

    IF Staining of MDCK using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

  • FC experiment of C2C12 using CL488-13938

    FC experiment of C2C12 using CL488-13938

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Calponin Polyclonal antibody (CL488-13938)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-13938

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

IF/ICC, IF-P, FC (Intra)

别名

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells, MDCK cells
Positive FC (Intra) detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 13938-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13938 targets Calponin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag4989

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC022015

Sequence: MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1
计算分子量 33 kDa
观测分子量 35 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
RRIDAB_2919081
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51911
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
IF protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols
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