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验证数据展示

  • WB analysis using 13938-1-AP

    WB analysis using 13938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13938-1-AP (Calponin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 13938-1-AP

    IHC staining of human heart using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 13938-1-AP

    IHC staining of human heart using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of mouse heart using 13938-1-AP

    WB analysis of mouse heart using 13938-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13938-1-AP (CNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse heart using 13938-1-AP

    IF Staining of mouse heart using 13938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Calponin antibody (13938-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using 13938-1-AP

    IF Staining of C2C12 using 13938-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse lung using 13938-1-AP

    IP experiment of mouse lung using 13938-1-AP

    IP result of anti-Calponin (IP:13938-1-AP, 4ug; Detection:13938-1-AP 1:1000) with mouse lung tissue lysate 2800ug.

  • IHC staining of human appendicitis using 13938-1-AP

    IHC staining of human appendicitis using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 13938-1-AP (Calponin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 13938-1-AP

    IF Staining of mouse heart using 13938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13938-1-AP (Calponin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of H9C2 using 13938-1-AP

    IF Staining of H9C2 using 13938-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of C2C12 using 13938-1-AP

    IF Staining of C2C12 using 13938-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of C2C12 using 13938-1-AP

    FC experiment of C2C12 using 13938-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Calponin (13938-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

Calponin Polyclonal antibody

Calponin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 13938-1-AP

产品说明书

引用文献(61)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse heart tissue, mouse lung tissue, mouse uterus tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman appendicitis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells, H9C2 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13938-1-AP targets Calponin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, bovine
免疫原

CatNo: Ag4989

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC022015

Sequence: MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1
计算分子量 33 kDa
观测分子量 35 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
RRIDAB_2082010
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

实验方案

Product Specific Protocols
IF protocol for Calponin antibody 13938-1-APDownload protocol
IHC protocol for Calponin antibody 13938-1-APDownload protocol
IP protocol for Calponin antibody 13938-1-APDownload protocol
WB protocol for Calponin antibody 13938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Circ Res

Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.

Authors - Felipe Paredes
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mouseWB

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
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ratIF

Acta Biomater

Hybrid electrospun rapamycin-loaded small-diameter decellularized vascular grafts effectively inhibit intimal hyperplasia.

Authors - Yang Yang
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mouseWB

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
View Article
ratWB

Phytomedicine

Betulinaldehyde inhibits vascular remodeling by regulating the microenvironment through the PLCγ1/Ca2+/MMP9 pathway

Authors - Yangxia Fu
View Article
ratWB

Phytomedicine

Quercetin improves airway remodeling in COPD rats by suppressing phenotypic switch of ASMCs via inhibiting the Wnt5a/β-catenin pathway

Authors - Hui Zhou
View Article
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