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验证数据展示

  • IF Staining of mouse colon using CL488-83793-5

    IF Staining of mouse colon using CL488-83793-5

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CoraLite® Plus 488 Cadherin-17 antibody (CL488-83793-5, Clone: 240681B11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using CL488-83793-5

    IF Staining of mouse colon using CL488-83793-5

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CoraLite® Plus 488 Cadherin-17 antibody (CL488-83793-5, Clone: 240681B11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Cadherin-17 Recombinant monoclonal antibody

Cadherin-17 Uni-rAb® Recombinant Antibody for IF-P
Cat No. CL488-83793-5

产品说明书

CloneNo. 240681B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

CDH17, Cadherin 17, CDH16, HPT 1, HPT1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse colon tissue
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83793-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83793-5 targets Cadherin-17 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19488

Product name: Recombinant human CDH17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 89-406 aa of BC113464

Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 17, LI cadherin (liver-intestine)
别名 CDH17, Cadherin 17, CDH16, HPT 1, HPT1
计算分子量 832 aa, 92 kDa
观测分子量120 kDa
GenBank蛋白编号BC113464
基因名称 Cadherin-17
Gene ID (NCBI) 1015
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12864
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cadherin-17 antibody CL488-83793-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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