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验证数据展示

  • WB analysis of mouse colon using 83793-5-RR

    WB analysis of mouse colon using 83793-5-RR

    mouse colon tissue was subjected to SDS PAGE followed by western blot with 83793-5-RR (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83793-5-RR

    IHC staining of human stomach cancer using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • IHC staining of human stomach cancer using 83793-5-RR

    IHC staining of human stomach cancer using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • IF Staining of human stomach cancer using 83793-5-RR

    IF Staining of human stomach cancer using 83793-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human stomach cancer using 83793-5-RR

    IF Staining of human stomach cancer using 83793-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human stomach cancer using 83793-5-RR

    IF Staining of human stomach cancer using 83793-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83793-5-RR

    Affinity and Kinetic Characterization of 83793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83793-5-RR against Human Cadherin-17 were performed. The affinity constant is below 1 pM.

Cadherin-17 Recombinant monoclonal antibody

Cadherin-17 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83793-5-RR

产品说明书

CloneNo. 240681B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman stomach cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83793-5-RR targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19488

Product name: Recombinant human CDH17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 89-406 aa of BC113464

Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 17, LI cadherin (liver-intestine)
别名 CDH17, HPT1, HPT 1, CDH16, Cadherin 17
计算分子量 832 aa, 92 kDa
观测分子量120 kDa
GenBank蛋白编号BC113464
基因名称 Cadherin-17
Gene ID (NCBI) 1015
RRIDAB_3671384
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ12864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

实验方案

Product Specific Protocols
IF protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
IHC protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
WB protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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