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验证数据展示

  • WB analysis using 20010-1-AP

    WB analysis using 20010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 20010-1-AP

    WB analysis of A549 using 20010-1-AP

    WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells.

  • IHC staining of human liver using 20010-1-AP

    IHC staining of human liver using 20010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 20010-1-AP

    IHC staining of human liver using 20010-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CYP4F2-Specific Polyclonal antibody

CYP4F2-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20010-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CPF2, CYP4F2, CYP4F2-Specific, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450 LTB omega

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HuH-7 cells, L02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20010-1-AP targets CYP4F2-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily F, polypeptide 2
别名 CPF2, CYP4F2, CYP4F2-Specific, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450 LTB omega
计算分子量 60 kDa
观测分子量 50-53 kDa
GenBank蛋白编号NM_001082
基因名称 CYP4F2
Gene ID (NCBI) 8529
RRIDAB_3085593
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.

实验方案

Product Specific Protocols
WB protocol for CYP4F2-Specific antibody 20010-1-APDownload protocol
IHC protocol for CYP4F2-Specific antibody 20010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols