验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HepG2 cells, SiHa cells, HeLa cells, Caco-2 cells. human placenta tissue, human testis tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67264-1-Ig targets CYP11A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4353 Product name: Recombinant human CYP11A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC032329 Sequence: MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 11, subfamily A, polypeptide 1 |
| 别名 | 1C5D5, Cholesterol desmolase, Cholesterol side-chain cleavage enzyme, mitochondrial, CYP11A, CYPXIA1 |
| 计算分子量 | 521 aa, 60 kDa |
| 观测分子量 | 49-52 kDa |
| GenBank蛋白编号 | BC032329 |
| 基因名称 | CYP11A1 |
| Gene ID (NCBI) | 1583 |
| RRID | AB_2882535 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05108 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| WB protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Neurosci Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats. | ||
Reprod Sci Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis | ||
J Ethnopharmacol Saffron extract alleviates D-gal-induced late-onset hypogonadism by activating the PI3K-Akt-Nrf2 signaling pathway | ||
Toxicol Appl Pharmacol Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice |
