验证数据展示
经过测试的应用
| Positive FC detected in | mouse thymocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-98233 targets CXCR4/CD184 in FC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | FC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2231 Product name: Recombinant Mouse CXCR4 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 1-40 aa of NM_009911.3 Sequence: MEPISVSIYTSDNYSEEVGSGDYDSNKEPCFRDENVHFNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) receptor 4 |
| 别名 | 241956H2, CD184, Cmkar4, C-X-C chemokine receptor type 4, Cxcr4 |
| 计算分子量 | 40kDa |
| GenBank蛋白编号 | NM_009911.3 |
| 基因名称 | Cxcr4 |
| Gene ID (NCBI) | 12767 |
| 偶联类型 | Biotin |
| 最大激发/发射波长 | - |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P70658 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CXCR4/CD184 antibody Biotin-98233 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
