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验证数据展示

  • WB analysis using 87890-1-RR

    WB analysis using 87890-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87890-1-RR (CWC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 87890-1-RR

    IF Staining of HeLa using 87890-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CWC15 antibody (87890-1-RR, Clone: 260028B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CWC15 Recombinant monoclonal antibody

CWC15 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87890-1-RR

产品说明书

CloneNo. 260028B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

AD-002, C11orf5, Cwf15, HSPC148, ORF5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSW 1990 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® CWC15 Ready-To-Use IHC Kit. CWC15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87890-1-RR targets CWC15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18116

Product name: Recombinant human CWC15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of BC040946

Sequence: MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CWC15 spliceosome-associated protein homolog (S. cerevisiae)
别名 AD-002, C11orf5, Cwf15, HSPC148, ORF5
计算分子量 229 aa, 27 kDa
观测分子量34 kDa
GenBank蛋白编号BC040946
基因名称 CWC15
Gene ID (NCBI) 51503
RRIDAB_3745809
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P013
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

实验方案

Product Specific Protocols
IF protocol for CWC15 antibody 87890-1-RRDownload protocol
WB protocol for CWC15 antibody 87890-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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