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验证数据展示

  • WB analysis using 87890-1-RR (same clone as 87890-1-PBS)

    WB analysis using 87890-1-RR (same clone as 87890-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87890-1-RR (CWC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87890-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87890-1-RR (same clone as 87890-1-PBS)

    IF Staining of HeLa using 87890-1-RR (same clone as 87890-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CWC15 antibody (87890-1-RR, Clone: 260028B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87890-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03260-1

    Cytometric bead array sample test of MP03260-1

    The mean CWC15 concentration was determined to be 7.5 ng/mL in Ramos cell extract based on a 1.2 mg/mL extract load, 5.7 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load, 7.9 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03260-1

    Cytometric bead array standard curve of MP03260-1

    Cytometric bead array standard curve of MP03260-1, CWC15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87890-1-PBS. Detection antibody: 87890-2-PBS. Standard: Ag18116. Range: 0.156-20 ng/mL.

CWC15 Recombinant monoclonal antibody, PBS Only (Capture)

CWC15 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87890-1-PBS

产品说明书

CloneNo. 260028B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

AD-002, C11orf5, Cwf15, HSPC148, ORF5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® CWC15 Ready-To-Use IHC Kit. CWC15 primary antibody included.

产品信息

87890-1-PBS targets CWC15 as part of a matched antibody pair:

MP03260-1: 87890-1-PBS capture and 87890-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18116

Product name: Recombinant human CWC15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of BC040946

Sequence: MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CWC15 spliceosome-associated protein homolog (S. cerevisiae)
别名 AD-002, C11orf5, Cwf15, HSPC148, ORF5
计算分子量 229 aa, 27 kDa
观测分子量34 kDa
GenBank蛋白编号BC040946
基因名称 CWC15
Gene ID (NCBI) 51503
RRIDAB_3745809
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P013
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

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