• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human PBMCs using 98078-1-RR

    FC experiment of human PBMCs using 98078-1-RR

    1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

  • FC experiment of human PBMCs using 98078-1-RR

    FC experiment of human PBMCs using 98078-1-RR

    1x10^6 PHA-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 unstimulated human PBMCs were surface stained with Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (98078-1-RR, Clone: 240793E8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • IHC staining of human tonsillitis using 98078-1-RR

    IHC staining of human tonsillitis using 98078-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 98078-1-RR

    IHC staining of human tonsillitis using 98078-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 98078-1-RR

    Affinity and Kinetic Characterization of 98078-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98078-1-RR

产品说明书

CloneNo. 240793E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

CTLA4, 240793E8, CD152, CELIAC3, CTLA 4

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98078-1-RR targets CTLA-4/CD152 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg0972

Product name: Recombinant Human CTLA4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 36-161 aa of BC070162

Sequence: KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytotoxic T-lymphocyte-associated protein 4
别名 CTLA4, 240793E8, CD152, CELIAC3, CTLA 4
计算分子量 223 aa, 25 kDa
GenBank蛋白编号BC070162
基因名称 CTLA4
Gene ID (NCBI) 1493
RRIDAB_3672225
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP16410
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.

实验方案

Product Specific Protocols
FC protocol for CTLA-4/CD152 antibody 98078-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...