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验证数据展示

  • WB analysis of THP-1 using 84827-2-RR (same clone as 84827-2-PBS)

    WB analysis of THP-1 using 84827-2-RR (same clone as 84827-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01609-3

    Sandwich ELISA standard curve of MP01609-3

    Sandwich ELISA standard curve of MP01609-3, Human G-CSFR Recombinant Matched Antibody Pair - PBS only. 84827-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2821. 84827-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP01609-3

    Sample test of MP01609-3

    The mean G-CSFR concentration was determined to be 7.76 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP01609-2

    Cytometric bead array standard curve of MP01609-2

    Cytometric bead array standard curve of MP01609-2, G-CSFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84827-2-PBS. Detection antibody: 84827-3-PBS. Standard: Eg2821. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01609-1

    Cytometric bead array standard curve of MP01609-1

    Cytometric bead array standard curve of MP01609-1, G-CSFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84827-1-PBS. Detection antibody: 84827-2-PBS. Standard: Eg2821. Range: 0.781-100 ng/mL.

G-CSFR Recombinant antibody, PBS Only (Capture/Detector)

G-CSFR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84827-2-PBS

产品说明书

CloneNo. 242246A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

G-CSF receptor, G CSF receptor, G CSF R, CSF3R, CD114

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84827-2-PBS targets G-CSFR as part of a matched antibody pair:

MP01609-1: 84827-1-PBS capture and 84827-2-PBS detection (validated in Cytometric bead array)

MP01609-2: 84827-2-PBS capture and 84827-3-PBS detection (validated in Cytometric bead array)

MP01609-3: 84827-4-PBS capture and 84827-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 3 receptor (granulocyte)
别名 G-CSF receptor, G CSF receptor, G CSF R, CSF3R, CD114
计算分子量92 kDa
观测分子量120-140 kDa
GenBank蛋白编号NM_000760.3
基因名称 G-CSFR
Gene ID (NCBI) 1441
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99062-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.