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验证数据展示

  • WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 30251-1-AP (same clone as 30251-1-PBS)

    IHC staining of mouse brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 30251-1-AP (same clone as 30251-1-PBS)

    IHC staining of rat brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 30251-1-AP (same clone as 30251-1-PBS)

    WB analysis of HCT 116 using 30251-1-AP (same clone as 30251-1-PBS)

    WB result of CRTAC1 antibody (30251-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAC1 transfected HCT 116 cells. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

CRTAC1 Polyclonal antibody, PBS Only

CRTAC1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30251-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

68 kDa chondrocyte-expressed protein, ASPIC, ASPIC1, Cartilage acidic protein 1, CEP 68

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30251-1-PBS targets CRTAC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32963

Product name: Recombinant human CRTAC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 198-332 aa of BC034245

Sequence: VVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDGKMVSRNVASGEMNSVLEILYPRDEDTLQDPAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cartilage acidic protein 1
别名 68 kDa chondrocyte-expressed protein, ASPIC, ASPIC1, Cartilage acidic protein 1, CEP 68
计算分子量 661 aa, 71 kDa
观测分子量 71-100 kDa
GenBank蛋白编号BC034245
基因名称 CRTAC1
Gene ID (NCBI) 55118
RRIDAB_3086281
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ79
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).

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